Pierce Anti-ha Agarose User Manual

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  1. Pierce Anti-ha Agarose User Manual 2017
  2. Pierce Anti-ha Agarose User Manual Pdf

Thermo Fisher anti ha magnetic beads Thermo Scientific Pierce Anti-HA Magnetic Beads are affinity particles for immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators. The anti-HA agarose has 3.5mg mouse anti-HA IgG1 monoclonal antibody conjugated permL of settled agarose resin. HA Synthetic Peptide (Product # 26184) is available for use in neutralization and control experiments and to competitively elute HA-tagged fusion proteins from immobilized anti-HA affinity resin.

Pierce Manufacturing publishes all operational manuals and a select number of service manuals online so that both firefighters and mechanics have access to the information needed. These manuals can be found online through Pierce’s website.

How to find them?

Pierce Anti-ha Agarose User Manual 2017

  1. Description HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available.
  2. May 16, 2017  Proteins of fewer than 50 amino acids are overlooked by most genomic and biochemical approaches and their functions are generally unknown. Here we report that a 31-amino acid inner membrane protein synthesized in response to limiting Mg2+ binds and stabilizes the major Mg2+ importer, thereby leading to increased intracellular levels of this critical ion.
  3. The Roles of Arabidopsis CDF2 in Transcriptional and Posttranscriptional Regulation of Primary MicroRNAs. With anti-HA-conjugated agarose. The pellet was analyzed by immunoblotting with anti-HA.
  4. Cell debris was pellet by centrifugation for 10 min at 15,000 rpm. The Co-IP experiments were performed using HA Tag IP/Co-IP Kit according to the manufacturer’s protocol (Thermo Pierce). Briefly, the mixed lysate was incubated with anti-HA agarose for 4 h to overnight in each spin column.

Pierce Anti-ha Agarose User Manual Pdf

  1. Click on the button to the right “PIERCE PARTS” or go to www.pierceparts.com to access Pierce’s parts website.
  2. Click the “Login as Guest” in the bottom left corner.
  3. Across the top is a tab identified as “Technical Support”.
  4. Scroll over “Technical Support” and a drop down menu will appear Identifying two new categories: “Publications” and “Supplier Website Links”
  5. Scroll over “Publications” and two new links will populate to the right: “Service Manuals” and “Operation’s Manuals”
  6. Select the manual category
    A) Click on “Operation’s Manuals” to open a sorted list of the operator’s manuals.
    B) Click on “Service Manuals” to access the limited amount of service manuals. There are a very select amount of service manuals available online. Reliant Fire has a more extensive list available to them. If the service manual desired is not published online, contact Reliant to see if one can be acquired.
  7. Click on the specific manual you desire and it will open up in PDF format for you to print or save.